| Name | Pos | Team | Age | Injured List |
|---|---|---|---|---|
| Francisco Álvarez | C | NYN | 23 | |
| Tyler Stephenson | C | Cin | 28 | |
| Jac Caglianone | 1B | KC | 22 | |
| Nick Kurtz | 1B | Oak | 22 | |
| Matt Mervis | 1B | Mia | 27 | |
| Ben Rice | 1B | NYA | 26 | |
| Michael Toglia | 1B | Col | 26 | |
| Spencer Torkelson | 1B | Det | 25 | |
| Gleyber Torres | 2B | Det | 28 | |
| CJ Abrams | SS | Was | 24 | |
| Adael Amador | SS (2B) | Col | N/A | |
| Elly De La Cruz | SS | Cin | 23 | |
| Gunnar Henderson | SS | Bal | 23 | |
| Junior Caminero | 3B | TB | 21 | |
| Mark Vientos | 3B | NYN | 25 | |
| Jordan Beck | OF | Col | 24 | |
| Jackson Chourio | OF | Mil | 21 | |
| Hunter Goodman | OF (C) | Col | 25 | |
| Trent Grisham | OF | NYA | 28 | |
| Trevor Larnach | OF | Min | 28 | |
| Brent Rooker | OF | Oak | 30 | |
| Kyle Schwarber | OF | Phi | 32 | |
| Zach Agnos | SP (RP) | Col | N/A | |
| Tanner Bibee | SP | Cle | 26 | |
| Jared Jones | SP | Pit | 23 | March 27, 2025:60 Day IL |
| Clayton Kershaw | SP | LAD | 37 | March 27, 2025:15 Day IL-Ab strain |
| Jack Leiter | SP | Tex | N/A | |
| Matthew Liberatore | SP | StL | 25 | |
| Casey Mize | SP | Det | 28 | |
| Paul Skenes | SP | Pit | 22 | |
| Jameson Taillon | SP | ChN | 33 | |
| Yoshinobu Yamamoto | SP | LAD | 26 | |
| Raisel Iglesias | RP | Atl | 35 | |
| Emilio Pagan | RP | Cin | 33 |