| Name | Pos | Team | Age | Injured List |
|---|---|---|---|---|
| Danny Jansen | C | TB | 30 | |
| Gabriel Moreno | C | Ari | 25 | |
| Luis Arraez | 1B | SD | 28 | |
| Willson Contreras | 1B | StL | 32 | |
| Yandy Diaz | 1B | TB | 33 | |
| Luke Keaschall | 2B | Min | 22 | April 26, 2025:10 Day IL |
| Otto Lopez | 2B | Mia | 26 | |
| Brandon Lowe | 2B | TB | 30 | |
| Zach Neto | SS | LAA | 24 | |
| Anthony Volpe | SS | NYA | 24 | |
| Matt Chapman | 3B | SF | 32 | |
| Jordan Westburg | 3B (2B) | Bal | 26 | April 28, 2025:10 Day IL |
| Roman Anthony | OF | Bos | 20 | |
| Corbin Carroll | OF | Ari | 24 | |
| Dylan Crews | OF | Was | 23 | |
| Jasson Dominguez | OF | NYA | 22 | |
| Tommy Edman | OF (2B) | LAD | 29 | |
| Lourdes Gurriel | OF | Ari | 31 | |
| Trevor Larnach | OF | Min | 28 | |
| Jackson Merrill | OF | SD | 22 | April 08, 2025:10 Day IL |
| Brent Rooker | OF | Oak | 30 | |
| Kris Bubic | SP | KC | 27 | |
| Corbin Burnes | SP | Ari | 30 | |
| Gerrit Cole | SP | NYA | 34 | March 22, 2025:60 Day IL |
| Jack Flaherty | SP | Det | 29 | |
| Sonny Gray | SP | StL | 35 | |
| Logan Henderson | SP | Mil | N/A | |
| Gunnar Hoglund | SP | Oak | 25 | |
| Nick Pivetta | SP | SD | 32 | |
| Pete Fairbanks | RP | TB | 31 | |
| Jeff Hoffman | RP | Tor | 32 | |
| Cade Smith | RP | Cle | N/A | |
| Robert Suarez | RP | SD | 34 | |
| Ryan Walker | RP | SF | N/A |