| Name | Pos | Team | Age | Injured List |
|---|---|---|---|---|
| Ryan Jeffers | C | Min | 27 | |
| Logan O'Hoppe | C | LAA | 25 | |
| Paul Goldschmidt | 1B | NYA | 37 | |
| Carlos Santana | 1B | Cle | 39 | |
| Ozzie Albies | 2B | Atl | 28 | |
| Kristian Campbell | 2B (OF) | Bos | 22 | |
| Matt McLain | 2B | Cin | 25 | |
| Mookie Betts | SS | LAD | 32 | |
| Ha-seong Kim | SS | TB | 29 | March 27, 2025:10 Day IL |
| Jordan Lawlar | SS | Ari | 22 | |
| Noelvi Marte | 3B | Cin | 23 | |
| Jose Ramirez | 3B | Cle | 32 | |
| Cam Smith | 3B (OF) | Hou | 22 | |
| Ronald Acuna Jr. | OF | Atl | 27 | March 25, 2025:10 Day IL |
| Charlie Condon | OF | Col | 22 | |
| Michael Conforto | OF | LAD | 32 | |
| Teoscar Hernández | OF | LAD | 32 | |
| Walker Jenkins | OF | Min | 20 | |
| Julio Rodriguez | OF | Sea | 24 | |
| Seiya Suzuki | OF | ChN | 30 | |
| Jordan Walker | OF | StL | 22 | |
| Spencer Arrighetti | SP | Hou | N/A | April 08, 2025:15 Day IL |
| Hunter Brown | SP | Hou | 26 | |
| Bubba Chandler | SP | Pit | N/A | |
| Clay Holmes | SP | NYN | 32 | |
| Jackson Jobe | SP | Det | 22 | |
| Merrill Kelly | SP | Ari | 36 | |
| Michael King | SP | SD | 29 | |
| Jesus Luzardo | SP | Phi | 27 | |
| Max Meyer | SP | Mia | 26 | |
| Drew Rasmussen | SP | TB | 29 | |
| Aroldis Chapman | RP | Bos | 37 | |
| Andres Munoz | RP | Sea | 26 | |
| Ryan Pressly | RP | ChN | 36 |