| Name | Pos | Team | Age | Injured List |
|---|---|---|---|---|
| Adley Rutschman | C | Bal | 27 | |
| Jonathan Aranda | 1B | TB | 26 | |
| Jac Caglianone | 1B | KC | 22 | |
| Bryce Eldridge | 1B | SF | 20 | |
| Ryan O'Hearn | 1B (OF) | Bal | 31 | |
| Matt Olson | 1B | Atl | 31 | |
| Wilmer Flores | 2B | SF | 33 | |
| Christian Moore | 2B | LAA | 22 | |
| Gabriel Arias | SS (2B,3B) | Cle | 25 | |
| Elly De La Cruz | SS | Cin | 23 | |
| Dansby Swanson | SS | ChN | 31 | |
| Coby Mayo | 3B | Bal | 23 | |
| Jorge Polanco | 3B | Sea | 31 | |
| Austin Riley | 3B | Atl | 28 | |
| Lawrence Butler | OF | Oak | 24 | |
| Kerry Carpenter | OF | Det | 27 | |
| TJ Friedl | OF | Cin | 29 | |
| Riley Greene | OF | Det | 24 | |
| Kameron Misner | OF | TB | N/A | |
| Jorge Soler | OF | LAA | 32 | |
| Matt Wallner | OF | Min | 27 | April 17, 2025:10 Day IL |
| Matt Boyd | SP | ChN | 34 | |
| Grant Holmes | SP | Atl | N/A | |
| Dustin May | SP | LAD | 27 | |
| Shane McClanahan | SP | TB | 28 | March 26, 2025:60 Day IL-Ab strain |
| Luis Ortiz | SP | Cle | 26 | |
| Cole Ragans | SP | KC | 27 | |
| Landen Roupp | SP | SF | N/A | |
| Tarik Skubal | SP | Det | 28 | |
| Tomoyuki Sugano | SP | Bal | 35 | |
| Gavin Williams | SP | Cle | 25 | |
| Brandon Woodruff | SP | Mil | 32 | March 27, 2025:15 Day IL |
| David Bednar | RP | Pit | 30 | |
| Emilio Pagan | RP | Cin | 33 |