| Position | Starters | Team | Opponents |
| C | Edgar Quero | ChA | @KC(4) Mia(3) |
| 1B | Pete Alonso | NYN | @Ari(3) ChN(3) |
| SS | Bobby Witt Jr. | KC | ChA(4) Bos(3) |
| 3B | Noelvi Marte | Cin | @Atl(4) @Hou(3) |
| OF | Pete Crow-Armstrong | ChN | SF(3) @NYN(3) |
| OF | Fernando Tatis Jr. | SD | @NYA(3) @Col(3) |
| DH | Shohei Ohtani | LAD | @Mia(3) @Ari(4) |
| Position | 1st Backup | Team | Opponents |
| 1B | Kyle Manzardo | Cle | @Was(3) Phi(3) |
| 2B (SS) | Jackson Holliday | Bal | @Min(3) @LAA(3) |
| SS | Jacob Wilson | Oak | Sea(3) NYA(3) |
| 3B | Rafael Devers | Bos | Tex(3) @KC(3) |
| OF | Dylan Crews | Was | Cle(3) StL(3) |
| DH | Jordan Beck | Col | Det(3) SD(3) |
| Position | 2nd Backup | Team | Opponents |
| 1B | Nick Kurtz | Oak | Sea(3) NYA(3) |
| DH | Caleb Durbin | Mil | Hou(3) @TB(3) |
| Position | 3rd Backup | Team | Opponents |
| SS | Sebastian Walcott | Tex | @Bos(3) @Det(3) |
| Position | Bench | Team | Opponents |
| 2B | Travis Bazzana | Cle | @Was(3) Phi(3) |
| 2B | Jeff McNeil | NYN | @Ari(3) ChN(3) |
| SS | Marcelo Mayer | Bos | Tex(3) @KC(3) |
| Pitching Staff Alignment |
| 5 Starts, 3 Relievers |
| Position | Pitching Order | Team | Opponents | Next Scheduled Start(s) |
| 1st SP | Roki Sasaki | LAD | @Mia(3) @Ari(4) | This Friday at Ari (Eduardo Rodriguez) |
| 2nd SP | Jackson Jobe | Det | @Col(3) Tex(3) | This Tuesday at Col (Chase Dollander) |
| 3rd SP | Clay Holmes | NYN | @Ari(3) ChN(3) | This Friday vs ChN (Jameson Taillon) |
| 4th SP | Zebby Matthews | Min | Bal(3) SF(3) | Has Not Played Yet |
| 5th SP | Clarke Schmidt | NYA | SD(3) @Oak(3) | This Tuesday vs SD (Michael King) |
| 6th SP (RP) | Luke Weaver | NYA | SD(3) @Oak(3) | Last Pitched Friday May 2nd |
| 7th SP | Tony Gonsolin | LAD | @Mia(3) @Ari(4) | This Tuesday at Mia (Cal Quantrill) |
| 8th SP | Jack Leiter | Tex | @Bos(3) @Det(3) | This Thursday at Bos (Tanner Houck) |
| 1st RP | Emmanuel Clase | Cle | @Was(3) Phi(3) | Last Pitched Saturday May 3rd |
| 2nd RP | Edwin Diaz | NYN | @Ari(3) ChN(3) | Last Pitched Saturday April 26th |
| 3rd RP | Tanner Scott | LAD | @Mia(3) @Ari(4) | Last Pitched Friday May 2nd |
| 4th RP | Luke Jackson | Tex | @Bos(3) @Det(3) | Last Pitched Wednesday April 30th |
| Position | Injured List | Team | Opponents |
| OF | Austin Hays | Cin | @Atl(4) @Hou(3) |
| 3B (2B) | Jordan Westburg | Bal | @Min(3) @LAA(3) |
| SP | Tyler Glasnow | LAD | @Mia(3) @Ari(4) |