| Name | Pos | Team | Age | Injured List |
|---|---|---|---|---|
| Tyler Heineman | C | Tor | 33 | |
| Salvador Perez | C (C,1B) | KC | 34 | |
| Joshua Bell | 1B | Was | 32 | |
| Paul Goldschmidt | 1B | NYA | 37 | |
| Nathaniel Lowe | 1B | Was | 29 | |
| Jake Cronenworth | 2B | SD | 31 | April 11, 2025:10 Day IL |
| Brendan Donovan | 2B (SS,OF) | StL | 28 | |
| Otto Lopez | 2B | Mia | 26 | |
| Willy Adames | SS | SF | 29 | |
| Geraldo Perdomo | SS | Ari | 25 | |
| Alex Bregman | 3B | Bos | 31 | |
| Eugenio Suarez | 3B | Ari | 33 | |
| Lourdes Gurriel | OF | Ari | 31 | |
| Aaron Judge | OF | NYA | 33 | |
| Josh Lowe | OF | TB | 27 | March 29, 2025:10 Day IL |
| Kameron Misner | OF | TB | N/A | |
| Andy Pages | OF | LAD | 24 | |
| Ceddanne Rafaela | OF | Bos | 24 | |
| Spencer Steer | OF (1B) | Cin | 27 | |
| Daulton Varsho | OF | Tor | 28 | |
| Marcell Ozuna | DH | Atl | 34 | |
| Griffin Canning | SP | NYN | 28 | |
| Hunter Dobbins | SP | Bos | N/A | |
| Jordan Hicks | SP | SF | 28 | |
| Merrill Kelly | SP | Ari | 36 | |
| Yusei Kikuchi | SP | LAA | 33 | |
| Seth Lugo | SP | KC | 35 | |
| Mitchell Parker | SP | Was | 25 | |
| Chris Sale | SP | Atl | 36 | |
| Brady Singer | SP | Cin | 28 | |
| Matt Waldron | SP | SD | 28 | March 27, 2025:15 Day IL |
| Kyle Finnegan | RP | Was | 33 | |
| Ryan Helsley | RP | StL | 30 | |
| Will Vest | RP | Det | 29 |