| Name | Pos | Team | Age | Injured List |
|---|---|---|---|---|
| Carson Kelly | C | ChN | 30 | |
| Shea Langeliers | C | Oak | 27 | |
| Gabriel Moreno | C | Ari | 25 | |
| Michael Busch | 1B | ChN | 27 | |
| Josh Naylor | 1B | Ari | 27 | |
| Spencer Torkelson | 1B | Det | 25 | |
| Nolan Gorman | 2B | StL | 24 | |
| Jackson Holliday | 2B (SS) | Bal | 21 | |
| Brooks Lee | 2B (3B) | Min | 24 | |
| Xander Bogaerts | SS | SD | 32 | |
| Trea Turner | SS | Phi | 31 | |
| Nolan Arenado | 3B | StL | 34 | |
| Alex Bregman | 3B | Bos | 31 | |
| Andrew Benintendi | OF | ChA | 30 | |
| Jackson Chourio | OF | Mil | 21 | |
| Dylan Crews | OF | Was | 23 | |
| Jasson Dominguez | OF | NYA | 22 | |
| Sal Frelick | OF | Mil | 25 | |
| Jesus Sanchez | OF | Mia | 27 | |
| Osvaldo Bido | SP | Oak | N/A | |
| Shane Bieber | SP | Cle | 29 | March 27, 2025:15 Day IL |
| Corbin Burnes | SP | Ari | 30 | |
| Max Fried | SP | NYA | 31 | |
| Zack Littell | SP | TB | 29 | |
| Tyler Mahle | SP | Tex | 30 | |
| David Peterson | SP | NYN | 29 | |
| Max Scherzer | SP | Tor | 40 | March 30, 2025:15 Day IL-Thumb |
| Trevor Williams | SP | Was | 33 | |
| Brandon Woodruff | SP | Mil | 32 | March 27, 2025:15 Day IL |
| Yoshinobu Yamamoto | SP | LAD | 26 | |
| Ryan Helsley | RP | StL | 30 | |
| Justin Martinez | RP | Ari | 23 | May 01, 2025:15 Day IL |
| Trevor Megill | RP | Mil | 31 | |
| Robert Suarez | RP | SD | 34 |