| Name | Pos | Team | Age | Injured List |
|---|---|---|---|---|
| Jonah Heim | C | Tex | 29 | |
| Logan O'Hoppe | C | LAA | 25 | |
| Joshua Bell | 1B | Was | 32 | |
| Vladimir Guerrero Jr. | 1B | Tor | 26 | |
| Wilmer Flores | 2B | SF | 33 | |
| Zack Gelof | 2B | Oak | 25 | March 23, 2025:15 Day IL |
| Orlando Arcia | SS | Atl | 30 | |
| Javier Baez | SS (3B,OF) | Det | 32 | |
| Zach Neto | SS | LAA | 24 | |
| Manny Machado | 3B | SD | 32 | |
| Dylan Moore | 3B (2B,OF) | Sea | 32 | April 29, 2025:10 Day IL |
| Austin Riley | 3B | Atl | 28 | |
| Ronald Acuna Jr. | OF | Atl | 27 | March 25, 2025:10 Day IL |
| Michael Harris II | OF | Atl | 24 | |
| Kameron Misner | OF | TB | N/A | |
| Cedric Mullins II | OF | Bal | 30 | |
| Chandler Simpson | OF | TB | 24 | |
| Lane Thomas | OF | Cle | 29 | April 22, 2025:10 Day IL |
| Daulton Varsho | OF | Tor | 28 | |
| Dylan Cease | SP | SD | 29 | |
| Erick Fedde | SP | StL | 32 | |
| George Kirby | SP | Sea | 27 | March 27, 2025:15 Day IL |
| Ryan Pepiot | SP | TB | 27 | |
| Freddy Peralta | SP | Mil | 28 | |
| Martin Perez | SP | ChA | 34 | April 19, 2025:60 Day IL |
| Colin Rea | SP (RP) | ChN | 34 | |
| Grayson Rodriguez | SP | Bal | 25 | March 27, 2025:60 Day IL |
| Chris Sale | SP | Atl | 36 | |
| Justin Steele | SP | ChN | 29 | April 09, 2025:60 Day IL |
| Spencer Strider | SP | Atl | 26 | April 21, 2025:15 Day IL |
| Logan Webb | SP | SF | 28 | |
| Kenley Jansen | RP | LAA | 37 | |
| Ryan Pressly | RP | ChN | 36 | |
| Will Vest | RP | Det | 29 |