| Name | Pos | Team | Age | Injured List |
|---|---|---|---|---|
| Ryan Jeffers | C | Min | 27 | |
| Joshua Bell | 1B | Was | 32 | |
| Tyler Soderstrom | 1B (OF) | Oak | 23 | |
| Jackson Holliday | 2B (SS) | Bal | 21 | |
| Luke Keaschall | 2B | Min | 22 | April 26, 2025:10 Day IL |
| Zach McKinstry | 2B (3B,OF) | Det | 30 | |
| Gleyber Torres | 2B | Det | 28 | |
| Bo Bichette | SS | Tor | 27 | |
| Geraldo Perdomo | SS | Ari | 25 | |
| Coby Mayo | 3B | Bal | 23 | |
| Mark Vientos | 3B | NYN | 25 | |
| Jordan Westburg | 3B (2B) | Bal | 26 | April 28, 2025:10 Day IL |
| Ronald Acuna Jr. | OF | Atl | 27 | March 25, 2025:10 Day IL |
| Byron Buxton | OF | Min | 31 | |
| Kerry Carpenter | OF | Det | 27 | |
| Dylan Crews | OF | Was | 23 | |
| Jasson Dominguez | OF | NYA | 22 | |
| Lourdes Gurriel | OF | Ari | 31 | |
| Jung Hoo Lee | OF | SF | 26 | |
| Marcell Ozuna | DH | Atl | 34 | |
| Kris Bubic | SP | KC | 27 | |
| Chase Burns | SP | Cin | 22 | |
| Logan Henderson | SP | Mil | N/A | |
| Gunnar Hoglund | SP | Oak | 25 | |
| Tylor Megill | SP | NYN | 29 | |
| Bailey Ober | SP | Min | 29 | |
| Grayson Rodriguez | SP | Bal | 25 | March 27, 2025:60 Day IL |
| Landen Roupp | SP | SF | N/A | |
| Joe Ryan | SP | Min | 28 | |
| Spencer Strider | SP | Atl | 26 | April 21, 2025:15 Day IL |
| Luke Weaver | SP (RP) | NYA | 31 | |
| Gavin Williams | SP | Cle | 25 | |
| Ryan Helsley | RP | StL | 30 | |
| Luke Jackson | RP | Tex | 33 |