| Name | Pos | Team | Age | Injured List |
|---|---|---|---|---|
| Joey Bart | C | Pit | 28 | |
| David Fry | C | Cle | 29 | February 19, 2025:60 Day IL |
| Gabriel Moreno | C | Ari | 25 | |
| Josh Naylor | 1B | Ari | 27 | |
| Andrés Gimenez | 2B | Tor | 26 | |
| Nico Hoerner | 2B | ChN | 27 | |
| Matt McLain | 2B | Cin | 25 | |
| Luis Rengifo | 2B (3B) | LAA | 28 | |
| Jon Berti | SS (2B,3B) | ChN | 35 | |
| Diego Castillo | SS | Min | 27 | |
| Anthony Volpe | SS | NYA | 24 | |
| Dylan Moore | 3B (2B,OF) | Sea | 32 | April 29, 2025:10 Day IL |
| Isaac Paredes | 3B | Hou | 26 | |
| Ronald Acuna Jr. | OF | Atl | 27 | March 25, 2025:10 Day IL |
| Roman Anthony | OF | Bos | 20 | |
| Teoscar Hernández | OF | LAD | 32 | |
| Garrett Mitchell | OF | Mil | 26 | April 26, 2025:10 Day IL |
| Julio Rodriguez | OF | Sea | 24 | |
| Juan Soto | OF | NYN | 26 | |
| Spencer Steer | OF (1B) | Cin | 27 | |
| Brayan Bello | SP | Bos | 25 | |
| Max Fried | SP | NYA | 31 | |
| George Kirby | SP | Sea | 27 | March 27, 2025:15 Day IL |
| Grayson Rodriguez | SP | Bal | 25 | March 27, 2025:60 Day IL |
| Roki Sasaki | SP | LAD | 23 | |
| Gavin Williams | SP | Cle | 25 | |
| Bryan Woo | SP | Sea | 25 | |
| Bryan Abreu | RP | Hou | 28 | |
| Camilo Doval | RP | SF | 27 | |
| Carlos Estevez | RP | KC | 32 | |
| Liam Hendriks | RP | Bos | 36 | |
| Kirby Yates | RP | LAD | 38 |