| Name | Pos | Team | Age | Injured List |
|---|---|---|---|---|
| Gabriel Moreno | C | Ari | 25 | |
| Bryce Eldridge | 1B | SF | 20 | |
| Christian Encarnacion-Strand | 1B | Cin | 25 | April 17, 2025:10 Day IL |
| Rhys Hoskins | 1B | Mil | 32 | |
| Luis Garcia | 2B | Was | 24 | |
| Jonathan India | 2B (3B,OF) | KC | 28 | |
| Gunnar Henderson | SS | Bal | 23 | |
| Trevor Story | SS | Bos | 32 | |
| Jace Jung | 3B | Det | 24 | |
| Josh Jung | 3B | Tex | 27 | |
| Andrew Benintendi | OF | ChA | 30 | |
| Lawrence Butler | OF | Oak | 24 | |
| Dylan Crews | OF | Was | 23 | |
| Adolis Garcia | OF | Tex | 32 | |
| Michael Harris II | OF | Atl | 24 | |
| Tyler O'Neill | OF | Bal | 29 | April 26, 2025:10 Day IL |
| Andy Pages | OF | LAD | 24 | |
| Bryan Reynolds | OF | Pit | 30 | |
| Victor Scott | OF | StL | 24 | |
| Chandler Simpson | OF | TB | 24 | |
| Tim Tawa | OF (2B) | Ari | N/A | |
| Mike Yastrzemski | OF | SF | 34 | |
| Jose Berrios | SP | Tor | 30 | |
| Ronel Blanco | SP | Hou | 31 | |
| Gerrit Cole | SP | NYA | 34 | March 22, 2025:60 Day IL |
| Tanner Houck | SP | Bos | 28 | |
| Tylor Megill | SP | NYN | 29 | |
| Aaron Nola | SP | Phi | 31 | |
| Robbie Ray | SP | SF | 33 | |
| Grayson Rodriguez | SP | Bal | 25 | March 27, 2025:60 Day IL |
| Max Scherzer | SP | Tor | 40 | March 30, 2025:15 Day IL-Thumb |
| Gavin Williams | SP | Cle | 25 | |
| Camilo Doval | RP | SF | 27 | |
| Robert Suarez | RP | SD | 34 |