| Name | Pos | Team | Age | Injured List |
|---|---|---|---|---|
| Agustin Ramirez | C | Mia | 23 | |
| Austin Wells | C | NYA | 25 | |
| Jac Caglianone | 1B | KC | 22 | |
| Nick Kurtz | 1B | Oak | 22 | |
| Kyle Manzardo | 1B | Cle | 24 | |
| Josh Naylor | 1B | Ari | 27 | |
| Zack Gelof | 2B | Oak | 25 | March 23, 2025:15 Day IL |
| Jackson Holliday | 2B (SS) | Bal | 21 | |
| Jeff McNeil | 2B | NYN | 33 | |
| Mookie Betts | SS | LAD | 32 | |
| Gunnar Henderson | SS | Bal | 23 | |
| Marcelo Mayer | SS | Bos | 22 | |
| Jacob Wilson | SS | Oak | 23 | |
| Junior Caminero | 3B | TB | 21 | |
| Caleb Durbin | 3B | Mil | 25 | |
| Cam Smith | 3B (OF) | Hou | 22 | |
| Corbin Carroll | OF | Ari | 24 | |
| Tommy Edman | OF (2B) | LAD | 29 | |
| Austin Hays | OF | Cin | 29 | May 02, 2025:10 Day IL |
| Chandler Simpson | OF | TB | 24 | |
| Fernando Tatis Jr. | OF | SD | 26 | |
| Shohei Ohtani | DH ,SP | LAD | 30 | |
| Garrett Crochet | SP | Bos | 25 | |
| Tyler Glasnow | SP | LAD | 31 | April 29, 2025:15 Day IL |
| Gunnar Hoglund | SP | Oak | 25 | |
| Clay Holmes | SP | NYN | 32 | |
| Grant Holmes | SP | Atl | N/A | |
| Jackson Jobe | SP | Det | 22 | |
| Landen Roupp | SP | SF | N/A | |
| Clarke Schmidt | SP | NYA | 29 | |
| Luke Weaver | SP (RP) | NYA | 31 | |
| José Alvarado | RP | Phi | 29 | |
| David Bednar | RP | Pit | 30 | |
| Emmanuel Clase | RP | Cle | 27 |