| Name | Pos | Team | Age | Injured List |
|---|---|---|---|---|
| Dillon Dingler | C | Det | N/A | |
| Shea Langeliers | C | Oak | 27 | |
| Josh Naylor | 1B | Ari | 27 | |
| Carlos Santana | 1B | Cle | 39 | |
| Kristian Campbell | 2B (OF) | Bos | 22 | |
| Romy Gonzalez | 2B (1B) | Bos | 28 | |
| Javier Baez | SS (3B,OF) | Det | 32 | |
| Bo Bichette | SS | Tor | 27 | |
| Xavier Edwards | SS | Mia | 25 | |
| Royce Lewis | 3B | Min | 25 | March 27, 2025:15 Day IL-Ab strain |
| Max Muncy | 3B | LAD | 34 | |
| Josh Smith | 3B (SS) | Tex | 27 | |
| Tommy Edman | OF (2B) | LAD | 29 | |
| TJ Friedl | OF | Cin | 29 | |
| Walker Jenkins | OF | Min | 20 | |
| Andy Pages | OF | LAD | 24 | |
| Luis Robert Jr. | OF | ChA | 27 | |
| Kyle Schwarber | OF | Phi | 32 | |
| Pavin Smith | OF (1B) | Ari | 29 | |
| Kyle Stowers | OF | Mia | 27 | |
| Christian Yelich | OF | Mil | 33 | |
| Ronel Blanco | SP | Hou | 31 | |
| Hunter Greene | SP | Cin | 25 | |
| George Kirby | SP | Sea | 27 | March 27, 2025:15 Day IL |
| David Peterson | SP | NYN | 29 | |
| Nick Pivetta | SP | SD | 32 | |
| Roki Sasaki | SP | LAD | 23 | |
| Shane Smith | SP | ChA | N/A | |
| Jeffrey Springs | SP | Oak | 32 | |
| Luke Weaver | SP (RP) | NYA | 31 | |
| Carlos Estevez | RP | KC | 32 | |
| Taijuan Walker | RP (SP) | Phi | 32 | |
| Devin Williams | RP | NYA | 30 |