| Name | Pos | Team | Age | Injured List |
|---|---|---|---|---|
| Joey Bart | C | Pit | 28 | |
| Logan O'Hoppe | C | LAA | 25 | |
| Jac Caglianone | 1B | KC | 22 | |
| Rhys Hoskins | 1B | Mil | 32 | |
| Josh Naylor | 1B | Ari | 27 | |
| Jake Cronenworth | 2B | SD | 31 | April 11, 2025:10 Day IL |
| Tyler Fitzgerald | 2B | SF | 27 | May 01, 2025:10 Day IL |
| Willy Adames | SS | SF | 29 | |
| Gabriel Arias | SS (2B) | Cle | 25 | |
| Kyren Paris | SS (2B,OF) | LAA | 23 | |
| Josh Jung | 3B | Tex | 27 | |
| Ryan McMahon | 3B | Col | 30 | |
| Joey Ortiz | 3B (SS) | Mil | 26 | |
| Yordan Alvarez | OF | Hou | 27 | |
| Roman Anthony | OF | Bos | 20 | |
| Byron Buxton | OF | Min | 31 | |
| Sal Frelick | OF | Mil | 25 | |
| Hunter Goodman | OF (C) | Col | 25 | |
| Trent Grisham | OF | NYA | 28 | |
| Ian Happ | OF | ChN | 30 | |
| Luis Robert Jr. | OF | ChA | 27 | |
| Victor Scott | OF | StL | 24 | |
| Andrew Abbott | SP | Cin | 25 | |
| Chris Bassitt | SP | Tor | 36 | |
| Taj Bradley | SP | TB | 24 | |
| Jacob deGrom | SP | Tex | 36 | |
| Kevin Gausman | SP | Tor | 34 | |
| Tyler Glasnow | SP | LAD | 31 | April 29, 2025:15 Day IL |
| Shane McClanahan | SP | TB | 28 | March 26, 2025:60 Day IL-Ab strain |
| Freddy Peralta | SP | Mil | 28 | |
| Tomoyuki Sugano | SP | Bal | 35 | |
| Jameson Taillon | SP | ChN | 33 | |
| Josh Hader | RP | Hou | 31 | |
| Raisel Iglesias | RP | Atl | 35 |