| Name | Pos | Team | Age | Injured List |
|---|---|---|---|---|
| Francisco Álvarez | C | NYN | 23 | |
| Dillon Dingler | C | Det | N/A | |
| J.T. Realmuto | C | Phi | 34 | |
| Vladimir Guerrero Jr. | 1B | Tor | 26 | |
| Nathaniel Lowe | 1B | Was | 29 | |
| Josh Naylor | 1B | Ari | 27 | |
| Luisangel Acuna | 2B | NYN | 23 | |
| Andrés Gimenez | 2B | Tor | 26 | |
| Nico Hoerner | 2B | ChN | 27 | |
| Bo Bichette | SS | Tor | 27 | |
| Xander Bogaerts | SS | SD | 32 | |
| Jose Caballero | SS (3B,OF) | TB | 28 | |
| Bobby Witt Jr. | SS | KC | 24 | |
| Alec Bohm | 3B | Phi | 28 | |
| Jace Jung | 3B | Det | 24 | |
| Isaac Paredes | 3B | Hou | 26 | |
| Cedric Mullins II | OF | Bal | 30 | |
| Bryan Reynolds | OF | Pit | 30 | |
| George Springer | OF | Tor | 35 | |
| Giancarlo Stanton | OF | NYA | 35 | March 26, 2025:60 Day IL |
| Daulton Varsho | OF | Tor | 28 | |
| Tyler Anderson | SP | LAA | 35 | |
| Griffin Canning | SP | NYN | 28 | |
| Jonathan Cannon | SP | ChA | N/A | |
| Kutter Crawford | SP | Bos | 29 | March 24, 2025:15 Day IL |
| Garrett Crochet | SP | Bos | 25 | |
| David Festa | SP | Min | N/A | |
| Rhett Lowder | SP | Cin | N/A | March 26, 2025:15 Day IL |
| Seth Lugo | SP | KC | 35 | |
| Aaron Nola | SP | Phi | 31 | |
| Colin Rea | SP (RP) | ChN | 34 | |
| Luis Severino | SP | Oak | 31 | |
| Justin Steele | SP | ChN | 29 | April 09, 2025:60 Day IL |
| Jameson Taillon | SP | ChN | 33 |