| Position | Starters | Team | Opponents |
| C | Miguel Amaya | ChN | SF(3) @NYN(3) |
| 1B | Josh Naylor | Ari | NYN(3) LAD(4) |
| 2B | Nico Hoerner | ChN | SF(3) @NYN(3) |
| SS | Bobby Witt Jr. | KC | ChA(4) Bos(3) |
| 3B | Eugenio Suarez | Ari | NYN(3) LAD(4) |
| OF | Julio Rodriguez | Sea | @Oak(3) Tor(3) |
| OF | Kyle Tucker | ChN | SF(3) @NYN(3) |
| OF | Christian Yelich | Mil | Hou(3) @TB(3) |
| DH | Jarren Duran | Bos | Tex(3) @KC(3) |
| Position | 1st Backup | Team | Opponents |
| C (OF) | Hunter Goodman | Col | Det(3) SD(3) |
| 1B (OF) | Ryan O'Hearn | Bal | @Min(3) @LAA(3) |
| 2B | Luis Garcia | Was | Cle(3) StL(3) |
| SS | Trea Turner | Phi | @TB(3) @Cle(3) |
| 3B (OF) | Cam Smith | Hou | @Mil(3) Cin(3) |
| OF | Jackson Merrill | SD | @NYA(3) @Col(3) |
| DH | Isaac Paredes | Hou | @Mil(3) Cin(3) |
| Position | 2nd Backup | Team | Opponents |
| C | Francisco Álvarez | NYN | @Ari(3) ChN(3) |
| 1B (2B) | Colt Keith | Det | @Col(3) Tex(3) |
| 2B | Travis Bazzana | Cle | @Was(3) Phi(3) |
| OF | Alex Verdugo | Atl | Cin(4) @Pit(3) |
| DH | Walker Jenkins | Min | Bal(3) SF(3) |
| Position | 3rd Backup | Team | Opponents |
| OF | Jorge Soler | LAA | Tor(3) Bal(3) |
| Pitching Staff Alignment |
| 8 Starts, 1 Reliever |
| Position | Pitching Order | Team | Opponents | Next Scheduled Start(s) |
| 1st SP | Casey Mize | Det | @Col(3) Tex(3) | This Wednesday at Col (Kyle Freeland) |
| 2nd SP | Michael King | SD | @NYA(3) @Col(3) | This Tuesday at NYA (Clarke Schmidt) |
| 3rd SP | David Peterson | NYN | @Ari(3) ChN(3) | This Tuesday at Ari (Zac Gallen) |
| 4th SP | Luis Castillo | Sea | @Oak(3) Tor(3) | This Friday vs Tor (Kevin Gausman) |
| 5th SP | Dustin May | LAD | @Mia(3) @Ari(4) | This Saturday at Ari (Corbin Burnes) |
| 6th SP | Clay Holmes | NYN | @Ari(3) ChN(3) | This Friday vs ChN (Jameson Taillon) |
| 7th SP | Grant Holmes | Atl | Cin(4) @Pit(3) | This Wednesday vs Cin (Hunter Greene) |
| 1st RP (SP) | Taijuan Walker | Phi | @TB(3) @Cle(3) | Last Pitched Thursday May 1st |
| 2nd RP | David Bednar | Pit | @StL(3) Atl(3) | Last Pitched Saturday May 3rd |
| Position | Injured List | Team | Opponents |
| SP | Spencer Strider | Atl | Cin(4) @Pit(3) |
| SP | Grayson Rodriguez | Bal | @Min(3) @LAA(3) |
| SP | Sean Manaea | NYN | @Ari(3) ChN(3) |